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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Nat Chem Biol. 2014 Mar 23;10(5):392–399. doi: 10.1038/nchembio.1494

Figure 6. Progression of pathway evolution throughout enzyme engineering stages.

Figure 6

(a) The original proposed three enzyme biosynthetic pathway. (b) The full pathway including the ATP regeneration cycle after identifying the kinase progenitor for phosphorylation of dideoxyribose and recognizing inhibition of PPM by nucleotide substrates and products. (c) The two step didanosine biosynthetic pathway as a result of the discovered bypass activity of the engineered RK. Dashed arrows indicate expected degradation products.