Figure 5. Apoptosis or cell death of semi-mature and mature CD4SP thymocytes in freshly isolated cells ex vivo or following in vitro culture.
(A) Apoptosis detected using 7AAD/Annexin V staining in freshly isolated Pak2F/F or Pak2F/F;Cd4-Cre CD4SP thymocytes. Semi-mature (CD69hiCD62Llow, Fraction A in Figure 4A), mature (CD69lowCD62Lhi, Fraction C in Figure 4A) and CD69lowCD62Llow (Fraction B in Figure 4A) CD4SP thymocytes were gated and analyzed. Data are representative of two independent experiments. (B) Intracellular staining of active caspase 3 following 0, 1, and 2 hr of incubation in 10% FBS serum containing media. Data are representative of two independent experiments. (C) Apoptosis detected using 7AAD/Annexin V staining following 24 hr of incubation in 10% FBS serum containing media. Semi-mature and mature CD4SP thymocytes were gated and analyzed. Data are representative of two independent experiments.