Figure 2. Metformin decreases cell proliferation by inhibiting mitochondrial complex I.
(A) Percentage of live Control-HCT 116 p53−/− or (B) NDI1-HCT 116 p53−/− treated with metformin for 72 hr in media containing 10 mM glucose. (C) Percentage of live Control-HCT116 p53−/− or (D) NDI1-HCT 116 p53−/− treated with metformin for 24 hr followed by glucose withdrawal for 16 hr. (E) Cell number of Control-HCT 116 p53−/− cells and (F) NDI1-HCT 116 p53−/− cells 24, 48, and 72 hr post treatment with 0.5 mM or 1 mM metformin in complete media. Error bars are SEM (n = 4). * indicates significance p<0.05.
Figure 2—figure supplement 1. Metformin decreases cellular proliferation through inhibition of mitochondrial complex I function in HCT 116 p53+/+ cells.
(A) Relative mitochondrial oxygen consumption rate of Control-HCT 116 p53+/+ cells and (B) NDI1-HCT 116 p53+/+ cells treated with metformin in complete media for 24 hr. (C) Cell number of Control-A549 cells and (D) NDI1-A549 cells 24, 48, and 72 hr post treatment with 0.5 mM or 1 mM metformin in complete media. Error bars are SEM (Relative OCR n = 3, Cell number n = 4). * indicates significance p<0.05.
Figure 2—figure supplement 2. Metformin decreases cellular proliferation through inhibition of mitochondrial complex I function in A549 cells.
(A) Relative mitochondrial oxygen consumption rate of Control-A549 cells and (B) NDI1-A549 cells treated with metformin in complete media for 24 hr. (C) Cell number of Control-A549 cells and (D) NDI1-A549 cells 24, 48, and 72 hr post treatment with 0.5 mM or 1 mM metformin in complete media. Error bars are SEM (Relative OCR n = 3, Cell number n = 4). * indicates significance p<0.05.