Fig. 2. Shortening of GOF18ΔPE-EGFP.
Four shortened forms (S1-S4) of the 17 kb long GOF18ΔPE-EGFP were generated by restriction endonuclease digestions: EGFP reporter with SV40 polyA termination, green box; distal enhancer (DE), orange box; Pou5f1 proximal promoter (PP), red box; Pou5f1 exons, open boxes.