Qualitative Analysis of ECM Proteins by Immunofluorescence Representative images
from triplicate lungs de-cellularized using each method and immunostained for
laminin (A–D), fibronectin (E–H), collagen IV (I–L),
collagen I (M–P), elastin (Q–T) qualitatively demonstrate loss
of ECM proteins from the de-cellularization process. The MP protocol appears to
retain the most laminin, fibronectin and Collagen IV compared to the CF and CP
protocols. The CP method appears to retain the most elastin. (All Images
63× Oil Magnification).