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Schematic of Re-cellularization of De-cellularized Scaffolds in Bioreactor System A physiologic heart lung bioreactor system from Harvard Apparatus was utilized to re-cellularize and culture C10-GFP cells on MP CF, and CP de-cellularized lung scaffolds. Following de-cellularization, cells were inoculated via the trachea (A) and allowed to adhere for 4 h. After that time, maintenance media was perfused through the pulmonary artery (B) and exited via the pulmonary vein (C) for 3 days.
