Summary of Rat Lung De-cellularization Methods De-cellularization reagent,
duration of protocol, levels of nuclear material still present after
de-cellularization and proliferation after re-seeding is summarized above. CP
de-cellularization appears to remove nucleic acids as well as the MP protocol in
a shorter period of time while using a single reagent. CF de-cellularization
appears to be ineffective in removing all nuclear material from the matrix
during this time point. Following re-seeding, cell proliferation was observed
only when utilizing the CP de-cellularized scaffold following 3 days in a
bioreactor. Abbreviations: 4′,6-diamidino-2-phenylindole (DAPI),
Propidium Iodide (PI), Sodium Deoxycholate (SDC), Sodium Chloride (NaCl), Sodium
Dodecyl Sulfate (SDS).