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. Author manuscript; available in PMC: 2014 May 12.
Published in final edited form as: J Immunol. 2010 Jul 16;185(4):2442–2449. doi: 10.4049/jimmunol.1000743

Figure 7. Impaired Th1 development in Salmonella-infected B7-H1-deficient mice.

Figure 7

Wild-type and B7-H1-deficient mice were infected IV with 5×105 attenuated Salmonella, BRD509. On day 6, 12, 21, 28, and 35, mice were injected with HKST for 4 hr and spleen cells analyzed for (A and B) intracellular IFN-γ production or (C and D) T-bet expression. (A) Representative FACS plots show IFN-γ and CD11a staining of CD4 T cells from naïve, day 6, day 12, day 28, and day 35, Salmonella-infected mice. (B) Pooled data showing the mean percentage ± SEM of CD11ahi IFN-γ-producing CD4 T cells in each group and represent at least three mice in each group. Mean IFN-γ production was significantly higher in Wt mice at days 12 and 21 by student t-test, *** p<0.005. (C) Representative histograms showing T-bet expression of CD4 T cells from naïve, day 6, day 10, day 20, and day 35, Salmonella-infected mice. (D) Pooled data showing the mean percentage ± SEM of T-bet expressing CD4 T cells from naïve or infected mice, and represent at least three mice in each group. Mean T-bet expression was significantly higher in Wt mice at days 6, 10, and 20 by student t-test, * p<0.05, *** p<0.005.