Table 1.
Tissue | Primary antibody, dilution | Secondary antibody, dilution | Dye, dilution |
---|---|---|---|
LMMP | Ms monoclonal neuronal HuC/D, 1:200 | Dk anti Ms IgG Dylight 488, 1:300 | NA |
Molecular Probes, Eugene, OR A21271 | Jackson ImmunoResearch, West Grove, PA 715-485-150 | ||
LMMP | Chk polyclonal GFAP, 1:1000 | Dk anti Chk IgY Dylight 594, 1:300 | NA |
Abcam, Cambridge, MA ab4674 | Jackson ImmunoResearch, West Grove, PA 703-515-155 | ||
LMMP | Gt polyclonal Sox10, 1:150 | Dk anti Gt IgG Dylight 594, 1:300 | NA |
SantaCruz Biotechnology, Santa Cruz, CA sc-17342 | Jackson ImmunoResearch, West Grove, PA 705-515-003 | ||
M | Rb polyclonal S100 protein, 1:400 | Biotinylated Gt anti Rb IgG, 1:300 | Streptavidin Cy3 conjugate, 1:600 |
DakoCytomatio, Glostrup Denmark Z0311 | Vector Labs, Burlingame, CA BA 1000 | Life Technologies, Grand Island, NY SA1010 |
LMMP: longitudinal muscle myenteric plexus preparation; M: full-thickness cryostat sections; Rb: Rabbit; Gt: Goat; Ms: Mouse; Dk: Donkey; Chk: Chicken.