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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Auton Neurosci. 2013 May 29;177(2):199–210. doi: 10.1016/j.autneu.2013.04.014

Table 1.

Antibody and manufacturer information for immunohistochemistry.

Tissue Primary antibody, dilution Secondary antibody, dilution Dye, dilution
LMMP Ms monoclonal neuronal HuC/D, 1:200 Dk anti Ms IgG Dylight 488, 1:300 NA
Molecular Probes, Eugene, OR A21271 Jackson ImmunoResearch, West Grove, PA 715-485-150
LMMP Chk polyclonal GFAP, 1:1000 Dk anti Chk IgY Dylight 594, 1:300 NA
Abcam, Cambridge, MA ab4674 Jackson ImmunoResearch, West Grove, PA 703-515-155
LMMP Gt polyclonal Sox10, 1:150 Dk anti Gt IgG Dylight 594, 1:300 NA
SantaCruz Biotechnology, Santa Cruz, CA sc-17342 Jackson ImmunoResearch, West Grove, PA 705-515-003
M Rb polyclonal S100 protein, 1:400 Biotinylated Gt anti Rb IgG, 1:300 Streptavidin Cy3 conjugate, 1:600
DakoCytomatio, Glostrup Denmark Z0311 Vector Labs, Burlingame, CA BA 1000 Life Technologies, Grand Island, NY SA1010

LMMP: longitudinal muscle myenteric plexus preparation; M: full-thickness cryostat sections; Rb: Rabbit; Gt: Goat; Ms: Mouse; Dk: Donkey; Chk: Chicken.