Table 1.
Primers used in the study
| Loci | Primers | Sequences | Annealing T° (time) | Expected size | Reference |
|---|---|---|---|---|---|
|
katG |
F |
5′-GAAACAGCGGCGCTGATCGT-3′ |
66°C (1 min) |
210 bp |
[21] |
| R |
5′- GTTGTCCCATTTCGTCGGGG- 3′ |
||||
|
fabGI-inhA |
F |
5′-CCTCGCTGCCCAGAAAGGGA-3′ |
64°C (1 min) |
248 bp |
[21] |
| R |
5′-ATCCCCCGGTTTCCTCCGGT-3′ |
||||
|
inhA (ORF) |
F |
5′- GAACTCGACGTGCAAAAC – 3′ |
55°C (45 sec) |
207 pb |
[18] |
| R |
5′- CATCGAAGCATACGAATA – 3′ |
||||
|
ahpC |
F |
5′-ACCACTGCTTTGCCGCCACC-3′ |
65°C (1 min) |
237 bp |
[21] |
| R |
5′-CCGATGAGAGCGGTGAGCTG-3′ |
||||
|
rpoB |
F |
5′-TCGCCGCGATCAAGGAGT-3′ |
62°C (30 sec) |
158 bp |
[21] |
| R |
5′-GTGCACGTCGCGGACCTCCA-3′ |
||||
|
rrs530 |
F |
5′-GATGACGGCCTTCGGGTTGT-3′ |
62°C (1 min) |
238 bp |
[12] |
| R |
5′- TCTAGTCTGCCCGTATCGCC -3′ |
||||
|
rrs912 |
F |
5′- GTAGTCCACGCCGTAAACGG -3′ |
62°C (1 min) |
240 bp |
[12] |
| R |
5′- AGGCCACAAGGGAACGCCTA -3′ |
||||
|
rpsL |
F |
5′- GGCCGACAAACAGAACGT -3′ |
58°C (30 sec) |
375 bp |
[12] |
| R |
5′- GTTCACCAACTGGGTGAC -3′ |
||||
|
embC |
F |
5′- GTTCGACAAGCGCGCCACAC -3′ |
65°C (45 sec) |
334 bp |
[22] |
| R |
5′- CGGAGGTAGATGGTAGCCGG -3′ |
||||
|
embA |
F |
5′- GCCGGCTATGTAGCCAACTA -3′ |
65°C (45 sec) |
338 bp |
[17] |
| R |
5′- GACCGTTCCACCAACACC -3′ |
||||
|
embB |
F |
5′- CCGACCACGCTGAAACTG -3′ |
65°C (45 sec) |
368 bp |
[23] |
| R |
5′- GTAATACCAGCCGAAGGGATCCT -3′ |
||||
|
gidB |
F |
5′-CGCCGAGTCGTTGTGCT-3′ |
62°C (1 min) |
886 pb |
- |
| R | 5′-AGCCTGGCCCGACCTTA-3′ |
T° = Temperature.