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Glioblastoma-derived endothelial cells. (a) Nuclei are stained with DAPI (blue), FISH EGFR probe (red) label tumor cells carrying EGFR amplification as double minutes (display multiple red signals). (b) Endothelial cells are detected by anti-CD31 immunofluorescence staining. ((c), (d)) A CD31+ (green) endothelial cell (arrow) carrying EGFR amplification (multiple red signals) is visible in a GBM microvessel.
