Figure 3.

Effects of canonical NF-κB signaling pathway on the proliferation of SCAPs. (a) Cell proliferation in NF-κB pathway-activated and control groups was detected by MTT assay. (b) Flow cytometry analysis for SCAPs in NF-κB pathway-activated and untreated groups. The proliferation index (PI = S% + G₂M%) in NF-κB pathway-activated group (26.52%) was significantly higher than that in control group (14.94%). (c) Images of scratch wounds in NF-κB pathway-activated and untreated groups at indicated time points. (d) Growth curves of NF-κB pathway-inhibited and untreated SCAPs. (e) Representative cell cycle distributions of NF-κB pathway-inhibited and untreated SCAP. The proliferation index (PI = S% + G₂M%) in NF-κB pathway-activated group (8.90%) was obviously lower than that in control group (17.83%). (f) Cell motility of SCAPs in NF-κB pathway-inhibited and untreated group assessed by a scratch assay. Values were the means ± SD; n = 6; *P < 0.05; **P < 0.01; ***P < 0.001. Scale bars = 100 μm.