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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Immunol. 2014 Mar 26;192(9):4436–4442. doi: 10.4049/jimmunol.1302909

Figure 2. SLAMF2 engagement by specific antibody activates dendritic cells.

Figure 2

IDCs and DNA-DCs were treated with 5 μg/ml of immobilized mouse control IgG or anti-SLAMF2 antibody (aSF2) for 24h. (A) Expression of cell surface activation molecules CD80, CD83 and CD86. (B) Production of pro-inflammatory cytokines IL-6 and TNFα. (C) MIP3-β-induced cell migration of DCs. (D) Gene expression of migration-related molecules CCR7, MMP-9 and TIMP-2. (E) IgG- or aSF2-pre-treated IDCs and DNA-DCs were co-cultured with total T cells for 24h (IL-2) or for 72h (IFNγ) and the cytokine levels were measured from supernatants. n=3, * p≤0.05; ** p≤0.01; *** p≤0.005