Figure 7. Dysregulation of dAdar auto-editing alters complex behaviors.

(a) Locomotor profile of dAdar^WTLoxP control males under 12 h light: 12 h dark (LD) conditions. Top panel – bar graph showing mean LD activity in dAdar^WTLoxP males (n = 65). Grey background indicates lights-off, white background indicates lights-on. Lower panel – mean locomotion in dAdar^S (n = 67) and dAdar^G (n = 77) males normalized to dAdar^WTLoxP. Light bars indicate s.e.m. (b) Locomotor profiles of dAdar^S and dAdar^G males. (c) Total locomotor levels derived from single-fly activity data. (d–e) Total locomotor levels (d) and relative climbing ability (e) derived from vertical population activity monitors. Climbing was quantified by normalizing the beams breaks in the top third of the vertical vial to the total number of beam breaks. dAdar^WTLoxP: n = 15 vials each containing 5 male flies; dAdar^S: n = 14; dAdar^G: n = 14. (f–g) Courtship in dAdar^WTLoxP, dAdar^S and dAdar^G males. Although the fraction of time spent courting females was not significantly different between experimental and control genotypes (f), dAdar^G males (n = 49) took significantly longer to initiate courtship relative to dAdar^WTLoxP (n = 41) or dAdar^S (n = 30) males (g). *: P < 0.05, **: P < 0.005, ***: P < 0.0005, one-way ANOVA with Tukey’s HSD post-hoc test. Error bars, s.e.m.