Figure 4.

A study to assess changes in the localization and/or distribution of actin regulatory proteins in human Sertoli cell following exposure to CdCl₂ or BPA. (A) The expression of several actin regulatory proteins, such as Apr3, c-Src and annexin II, examined by immunoblotting of lysates of human Sertoli cells was not significantly affected by CdCl₂ or BPA unless a high dose was used (see left panel). These immunoblotting data are summarized in the histogram shown on the right panel. Each bar is a mean ± SD of n = 3 independent experiments. *P < 0.05; **P < 0.01 with treatment versus corresponding control (Ctrl) group. (B) Sertoli cells were exposed to either CdCl₂ (5 or 20 µM) or BPA (40 or 200 µM) for 2 days before being fixed and processed for immunofluorescence analysis to visualize the distribution and/or localization of Arp3, F-actin, Eps8, Src and annexin II. Cell nuclei were visualized by DAPI staining. Scale bar = 40 µm, which applies to all other micrographs.