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. 2014 Feb 13;29(6):1279–1291. doi: 10.1093/humrep/deu011

Table I.

Human occludin-specific primers used for RT–PCRa.

Primer sequence Orientation Position Size (bp) Tm (°C) Reference
5′-GACCCAAGAGCAGCAA-3′ Sense 833–848 688 50.5 NC_018921.2/NM_001205254.1
5′-CATCCACAGGCGAAGT-3′ Antisense 1505–1520
5′-TTTGTGGGACAAGGAACACA-3′ Sense 1273–1292 312 53.8 NC_018921.2/NM_001205255.1
5′-GCAGGTGCTCTTTTTGAAGG-3′ Antisense 1565–1584

aRT–PCR was performed by using a GeneAmp 2400 PCR system (Applied Biosystems, Inc.), Tm (°C), annealing temperature and 30 cycles.