Table I.
Primer sequence | Orientation | Position | Size (bp) | Tm (°C) | Reference |
---|---|---|---|---|---|
5′-GACCCAAGAGCAGCAA-3′ | Sense | 833–848 | 688 | 50.5 | NC_018921.2/NM_001205254.1 |
5′-CATCCACAGGCGAAGT-3′ | Antisense | 1505–1520 | |||
5′-TTTGTGGGACAAGGAACACA-3′ | Sense | 1273–1292 | 312 | 53.8 | NC_018921.2/NM_001205255.1 |
5′-GCAGGTGCTCTTTTTGAAGG-3′ | Antisense | 1565–1584 |
aRT–PCR was performed by using a GeneAmp 2400 PCR system (Applied Biosystems, Inc.), Tm (°C), annealing temperature and 30 cycles.