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. Author manuscript; available in PMC: 2014 May 12.
Published in final edited form as: J Mol Biol. 2013 Feb 20;425(11):1961–1981. doi: 10.1016/j.jmb.2013.02.017

Figure 9. Effect of Mg2+ on the kinetics of inactivation of FVa by APC.

Figure 9

Percent FVa residual activity is plotted against time. Inactivation was monitored by the decrease in FVa clotting activity as described in ″Materials and Methods″. APC was 8 nM, FVa was 120 nM and phospholipid was 10 µM. The reaction was carried out at 37 °C in buffer containing various concentrations Ca2+ (panel A, 0.5 mM Ca2+; panel B, 0.8 mM Ca2+; panel C, 1.1 mM Ca2+; and panel D, 2 mM Ca2+) in the absence (○) or presence of 0.6 mM Mg2+ (●). The results presented are the average of three experiments.