Table 2.
Conditions* | ||||
---|---|---|---|---|
Ca2+ mM |
Mg2+ mM |
kon M−1s−1 |
koff s−1 |
Kd µM |
1.1 | 0.0 | 1.1 × 103 | 3.2 × 10−3 | 2.9 |
1.7 | 0.0 | 1.5 × 103 | 3.1 × 10−3 | 2.1 |
1.1 | 0.6 | 1.9 × 103 | 3.2 × 10−3 | 1.7 |
In the presence of 5 mM Ca2+, kon, koff and Kd values were similar to those obtained under the physiological Ca2+/Mg2+ (1.1 mM Ca2+/0.6 mM Mg2+) condition (data not shown).