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. Author manuscript; available in PMC: 2014 May 12.
Published in final edited form as: J Mol Biol. 2013 Feb 20;425(11):1961–1981. doi: 10.1016/j.jmb.2013.02.017

Table 2.

The effect of Mg2+ on D-FFR-VIIa binding to PC/PS phospholipid bilayer

Conditions*
Ca2+
mM		 Mg2+
mM		 kon
M−1s−1 		koff
s−1 		Kd
µM
1.1 0.0 1.1 × 103 3.2 × 10−3 2.9
1.7 0.0 1.5 × 103 3.1 × 10−3 2.1
1.1 0.6 1.9 × 103 3.2 × 10−3 1.7
*

In the presence of 5 mM Ca2+, kon, koff and Kd values were similar to those obtained under the physiological Ca2+/Mg2+ (1.1 mM Ca2+/0.6 mM Mg2+) condition (data not shown).