Table 1. Initial Occupancies of Each Subpopulation under Each Experimental Condition of Temperature and Excitation Pulse Energya.
photoactive
population occupancy (%) |
fluorescent
population occupancy (%) |
||||||
---|---|---|---|---|---|---|---|
Pr 1 | Pr 2 | total | Pr 3 | Pr 4 | Pr 5 | total | |
temperature dependence (630 nm excitation) | |||||||
8 °C | 25 | 48 | 73 | 4 | 17 | 6 | 27 |
25 °C | 16 | 48 | 64 | 8 | 21 | 7 | 36 |
40 °C | 13 | 40 | 53 | 11 | 29 | 7 | 47 |
excitation energy dependence (at 25 °C) | |||||||
600 nm excitation | 20 | 32 | 52 | 5 | 38 | 5 | 48 |
670 nm excitation | 35 | 40 | 75 | 3 | 18 | 5 | 26 |
The five subpopulations, Pr 1–5, are categorized as photoactive (Pr 1 and 2) and fluorescent (Pr 3–5) populations. These parameters are based on the target model from Figure 5.