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. 2010 Oct 25;2(1):53–57. doi: 10.1021/ml1002053

Figure 2.

Figure 2

GPR54 agonist-mediated activation of NPFFRs. (a and b) Intracellular Ca2+ mobilization in HEK293T cells expressing NPFFR1 (a) or NPFFR2 (b). The intracellular Ca2+ response was calculated from the maximum fluorescence intensity after the addition of neuropeptide AF (NPAF; 6 μM for NPFFR1 and 1.2 μM for NPFFR2). (c and d) GPR54 agonist-mediated inhibition of forskolin-induced cAMP production in CHO cells expressing NPFFR1 (c) or NPFFR2 (d). The cAMP level was calculated from the maximum signal after the addition of NPSF (100 μM) using an AlphaScreen cAMP detection system. The data are expressed as the mean ± SD (N = 3).