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Dose-dependent production of nitrite in HT29-dx cells treated with DOXO and NO−DOXOs. Cells were incubated for 24 h in the absence or presence of different concentrations (0.5, 1, 2.5, 5, and 25 μM) of DOXO (open columns), 4 (hatched columns), or 5 (solid columns), and then, the levels of nitrite in the cell medium were evaluated by the Griess method. Data are presented as means ± SDs (n = 3) vs untreated cells (0): *p < 0.001.
