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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Cytometry A. 2013 Oct 17;83(12):1096–1104. doi: 10.1002/cyto.a.22401

Figure 1. PMA/Ionomycin concentration-dependent stimulation of NFAT1 cytosolic to nuclear translocation quantified by imaging flow cytometry.

Figure 1

Jurkat T cell line treated with PMA/Ionomycin for 30 minutes at 37°C, 5% CO2. Nuclear localization of NFAT1 with stimulation is observed as indicated by a shift in similarity score distribution to the right (Figure A). Whole blood collected from healthy donors and 500 μL stimulated with increasing dilutions PMA/Ionomycin for 30 minutes. A dilution-dependent decrease in activation in CD4+ and CD8+ T cells is observed (Figure B). Results represent the mean and standard error of 2 independent experiments. Representative images of both unstimulated and stimulated populations are also shown with their corresponding Similarity score (SS) (Figure C).