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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Cytometry A. 2013 Oct 17;83(12):1096–1104. doi: 10.1002/cyto.a.22401

Figure 2. PMA/Ionomycin-induced NFAT1 nuclear localization precedes increase in downstream cytokine expression.

Figure 2

Whole blood collected from healthy donors and 500 μL stimulated with PMA/Ionomycin for 0 – 24 hours with 2.5 μg/mL Brefeldin A to promote protein accumulation within the cell. NFAT1 translocation (grey bars, left axis) peaks within 0.5 hours stimulation, followed by an accumulation of its downstream target protein, IFNγ (black bars, right axis), which peaks at 24 hours when NFAT1 has returned to the cytoplasm. Results represent the mean and standard error of 4 independent experiments (*p<0.05, **p<0.01; Students t test).