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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Cytometry A. 2013 Oct 17;83(12):1096–1104. doi: 10.1002/cyto.a.22401

Figure 4. Peak plasma levels of tacrolimus from renal transplant recipients can inhibit NFAT1 nuclear translocation in Jurkat T cell line treated ex vivo.

Figure 4

Whole blood collected from 3 renal transplant recipients (A-C) at tacrolimus trough (time zero) and over the first 6 hours post-tacrolimus administration and plasma harvested ex vivo. Jurkat T cells were suspended in patient plasma at 37°C, 5%CO2 for 2 hours; at 1.5 hours cells were stimulated with PMA/Ionomycin for remaining 30 minutes at 37°C, 5%CO2. A time dependent inhibition of NFAT1 translocation (black line, left axis) was found between 1 to 4 hours and corresponds with the peak levels of tacrolimus in whole blood (grey line, left axis) measured by immunoassay.