Skip to main content
. Author manuscript; available in PMC: 2015 Apr 24.
Published in final edited form as: Chem Biol. 2014 Mar 20;21(4):453–458. doi: 10.1016/j.chembiol.2014.02.011

Figure 3.

Figure 3

Effect of bosutinib on the allosteric activator function of HER3 in vitro. Concentration dependent effect of bosutinib on measured kinase activity in the following bosutinib binding modalities: (A) HER3 and EGFR binding (HER3 and EGFR-V924R, orange squares), (B) HER3 binding only (HER3 and EGFR-V924R/T766M, purple circles), (C) no binding (HER3-T768I and EGFR-V924R/T766M, green triangles). The small black star on the EGFR kinase domain represents the V924R mutation preventing self-activation. (D) Normalized kinase activity in response to bosutinib titration. Error bars in all plots represent SD of two independent measurements. ** P < 0.001.