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. Author manuscript; available in PMC: 2015 Apr 24.
Published in final edited form as: Chem Biol. 2014 Mar 20;21(4):453–458. doi: 10.1016/j.chembiol.2014.02.011

Figure 4.

Figure 4

JM-EGFR kinase activity measured under titration with JM-HER3. (A) Kinase activity measured when JM-EGFR and JM-HER3 constructs were concentrated on a membrane surface. Left plot shows observed reaction rates, while right plot shows the fold enhancement in kinase activity produced by addition of bosutinib. (B) As in (A), for kinase activity measured in the absence of lipid membranes. JM-HER3 concentrations in all panels reflect the bulk solution concentration. The concentration of JM-EGFR was held constant in all experiments. Bosutinib concentrations were equal to the listed JM-HER3 concentration plus an additional 2 uM excess. Error bars in all plots represent SD of two independent measurements.