Skip to main content
. 2014 May 12;9(5):e97229. doi: 10.1371/journal.pone.0097229

Figure 4. Kinetics of gp120-specific antibody titers and innate immune recruiting properties of antibodies following SHIV-SF162P3 challenge.

Figure 4

ELISA was used to test the level of gp120-specific antibodies in the plasma of infected rhesus macaques (A) that received purified antibodies from either the pool of HIV-negative individuals (i) or the elite controller (ii). Optical Density (OD) values are reported. The level of gp120 specific antibody subclass over time was measured by Luminex in the plasma of the 4 infected monkeys that received the EC antibodies as well as in one monkey that received the HIV-NEG antibodies and is reported as mean fluorescence intensity (B–E). Antibodies were purified from the rhesus monkeys that received the EC antibodies (i) or the HIV-NEG antibodies (ii) and tested for their ability to recruit NK cells to mediate ADCC (F) or to recruit monocytes to mediate phagocytosis (G) 2 weeks prior the antibody transfer and at day 1, 3, 7, 14, 21 and 28 post antibody transfer. NK cells isolated from 2 separate donors were used to assess ADCC and 2 separate experiments were performed to measure the phagocytic activity.