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. 2014 May 12;9(5):e97174. doi: 10.1371/journal.pone.0097174

Figure 2. Attenuation of PSPC1 expression inhibits cell proliferation.

Figure 2

(A) HeLa cells were transfected with 40 nM PSPC1 siRNAs (siPSPC1) or control siRNA (siControl) (‘Materials and Methods’ section). 24 h later, expression of PSPC1 was analyzed using quantitative real-time PCR (left histogram) and Western blot (right panels). β-actin was used as the loading control. (B) Cell proliferation of HeLa cells transfected with siPSPC1 or siControl was measured by the Trypan blue exclusion assay. Left, total cell number; Right, viable cell number. Data represents the average of three independent experiments with six replicate measurements (mean ± SD).