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. 2014 May 12;9(5):e97174. doi: 10.1371/journal.pone.0097174

Figure 4. Alteration of PSPC1 expression influences the formation of γH2AX foci.

Figure 4

HeLa cells were transfected with siPSPC1 or siControl. 24-transfection, cells were treated with 2.5 or 5 µM of cisplatin for 12 h, and the expression of γH2AX was examined by Western blot (A), flow cytometry (B), and immunofluorescence microscopy (C). (D) HeLa cells were transfected with either pPSPC1 or pCON to overexpress PSPC1. 24 h post-transfection, cells were treated with 5 µM of cisplatin for 12 h, and the expression of γH2AX or PSPC1 was examined by Western blot. *P< 0.05, compared with control.