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. 2014 May 12;9(5):e97174. doi: 10.1371/journal.pone.0097174

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© 2014 Gao et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) PSPC1 does not form distinct foci with γH2AX, 53BP1 or Rad51. Representative confocal laser scanning images of HeLa cells were analyzed 12 h after 5 µM cisplatin treatment. (B) DNA repair kinetic curve in siControl and siPSPC1 cells as calculated by the intensity of γH2AX measured by immunofluorescence microscopy. Quantitative analysis of average density (Fluorescence intensity per unit area) was determined by Image-Pro Plus 6.0.