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. 2014 May 12;9(5):e97174. doi: 10.1371/journal.pone.0097174

Figure 6. Loss of PSPC1 causes cells to enter G2/M phase.

Figure 6

(A) HeLa cells were cultured in the presence of 2 mM thymidine for 18 h, washed with PBS, and transfected with siRNAs using Lipofectamine2000. 8 h after transfection, thymidine was added again to 2 mM to block cells at the G1/S boundary. After another 18 h, cells were transferred to fresh medium for 24 h, then harvested and analyzed by flow cytometry. (B) Using the same cells, the levels of representative G2/M phase proteins (phospho-histone H3 (Ser10), Cdc2 and cyclinB [55]) were examined by Western blot.