Table 2. Efficiencies of Q/R RNA editing of gria2α.
Genotypesa | Developmental stages (hpf) | Normalized gria2αR frequencyb (sample number) |
Wild type + Adar2 | 12 | 128.57% (2) |
adar2MOc | 12 | 103.93±2.03% (3) |
adar2MOsp | 12 | 91.72±4.31%* (5) |
adar2MOt | 12 | 75.82±18.33%* (5) |
adar2MOt + Adar2 | 12 | 135.86±25.07%** (4) |
adar2MOt + Adar2cd | 12 | 55.05±25.07%* (4) |
gria2αQRMO | 12 | 37.25±32.2%* (3) |
gria2αQRMO + Adar2 | 12 | 25.04% (2) |
adar2MOc | 24 | 102.94±2.55% (3) |
adar2MOsp | 24 | 69.81±18.74%* (4) |
adar2MOsp + Adar2 | 24 | 82. 2% (1)c |
adar2MOt | 24 | 68.93±18.86%* (8) |
Adar2MOt + Adar2 | 24 | 66.09% (1)d |
adar2MOt-p53 AUG | 24 | 75.71±11.48%* (3) |
gria2αQRMO | 24 | 18.59±11.43%* (6) |
gria2αQRMO-p53 AUG | 24 | 30.49±8.77%* (3) |
adar2MOt | 48 | 71.62 ±17.13% (5) |
gria2αQRMO | 48 | 25.02±6.31% (3) |
: morphants and protein expressed from the injected cRNA.
: The fractions of edited gria2αR in the total gria2α transcript were determined and then normalized to that of the un-injected (wild type) embryos of the same batch. The fractions of gria2αR present in the 12-hpf wild type embryos varied from 50% to 65%, while it was between 96 to 98% in the 24-hpf and 48-hpf wild type embryos [18]. Values are represented as means ± standard deviation (number of independent injection). Statistics was analyzed by Student's t-test. Asterisk (*) indicates a significant difference (p<0.05) versus adar2MOc. Double asterisks indicate a significant difference versus adar2MOt.
: the gria2αR frequency of the corresponding adar2MOsp was 81%.
: the gria2αR frequency of the corresponding adar2MOt was 72%.