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. Author manuscript; available in PMC: 2014 May 12.
Published in final edited form as: Methods Mol Biol. 2013;960:545–555. doi: 10.1007/978-1-62703-218-6_40

Fig. 1.

Fig. 1

Summary of methods 3.2 & 3.3, generating CD4+ T cell clones and cell lines. A: After primary stimulation of PBMC stain cultures with tetramer and antibody. B: Single cell sort CD4+ tetramer positive cells into a 96-well plate to generate a clone or bulk the cells into a sterile capped FACS tube and plate to generate a cell line. C: After stimulation and expansion test antigen specificity of cells with tetramer staining, as well as proliferation assays and cytokine staining (not shown).