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. 2014 May 12;9(5):e97440. doi: 10.1371/journal.pone.0097440

Figure 7. Fluorescence emission spectrum of EGFP before and after photodepletion.

Figure 7

The initial spectrum (black line) before photodepletion is virtually identical to the spectrum (red line) taken at a photodepletion fraction of 0.87. Both spectra have been scaled to a maximum amplitude of one to facilitate visual comparison.