Figure 6. Effect of cycloheximide and KCl on localisation of eIF4G and SUMO.

Cells containing eIF4G-HA, untreated (UT) or exposed to CHX (100 µg/ml) or KCl (1 M) as indicated, were incubated with anti-SUMO antisera (green) and anti-HA antisera (red). Bar  = 5 µm. Bottom panel, regions indicated by boxes in panel above. Arrows indicate sites of colocalisation of SUMO and eIF4G.