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. 2014 May 12;9(5):e96957. doi: 10.1371/journal.pone.0096957

Figure 1. Caco-2 cells are susceptible to TBEV infection.

Figure 1

Human cells infected with TBEV strains K23, Sofjin, and Ania at an MOI of 0.1. Viral supernatant and intracellular viral RNA were harvested at 24(A) Intracellular TBEV RNA copy numbers, measured by RT-qPCR. (B) Viral titers in the supernatant determined by plaque assay, n = 3; *P<0.05, **P<0.01 to initial virus titer in Student's t test. (C) Immunofluorescence assay of TBEV-infected Caco-2 cell monolayers. Caco-2 cells infected with TBEV K23 strain were fixed at different time points and subjected to immunofluorescence assay. TBEV E (green), nuclei (blue, DAPI = 4′-6-diamidino-2-phenylindole dihydrochloride). One representative image of a triplicate is shown.