Fig. 5.

GSK3β inhibition attenuated oxidant stress-induced hepatocyte apoptosis. a LDH activities assay in the HL-7,702 cell culture supernatant. Positive control group: the maximum LDH release induced by the addition of 1× lysis solution. Negative control group: the LDH activity released from the untreated normal cells. SB216763 group: the LDH activity released from the solely SB216763-treated for 12 h. H₂O₂ group and SB263763+ H₂O₂ group: cells were pre-incubated with or without SB216763 (10 mΜ) for 2 h, then incubated H₂O₂ (1 mmol/L) for 12 h. Antimycin A group and SB263763+ Antimycin A group: cells were pre-incubated with or without SB216763 (10 mΜ) for 2 h, then incubated with Antimycin A (2 μg/ml) for 12 h. All cells in the groups are in the 48 wells culture plates (3 wells/group). *p < 0.05 compared with H₂O₂-treated cells; ^# p < 0.05 compared with Antimycin A-treated cells. b The expression of caspas-3, cleaved caspase-3, p-JNK, total JNK, Cyt c and β-actin proteins was measured by western blot. Pre-incubated with or without SB216763 (10 mΜ) for 2 h, then HL-7,702 cells are stimulated with H₂O₂ (1 mmol/L) or Antimycin (2 μg/ml) for 12 h. The control group is the untreated normal cells. Cell lysates were subject to western blot with antibodies. Representatives of experiments are shown