Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 May 1;28(9):1005–1017. doi: 10.1101/gad.238709.114

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Fantauzzo and Soriano; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 5. — PI3K-engaged PDGFRα signaling mediates cell survival and proliferation in primary MEPM cells. (A) Cell growth as assessed by crystal violet staining in E13.5 primary MEPM cells grown in medium containing 10% FBS and treated once with LY294002 or H₂O₂ and/or daily with PDGF-AA for up to 4 d. MEPM cell survival is impaired in the presence of the PI3K inhibitor LY294002 (64.88% ± 1.538% decrease in relative absorbance; P = 0.0130) regardless of PDGF-AA ligand stimulation (68.48% ± 0.2966% decrease in relative absorbance; P = 0.0197). PDGF-AA treatment provides protection from hydrogen peroxide-induced apoptosis (54.88% ± 26.34% increase in relative absorbance; P = 0.1653). (B) Cell growth as assessed by crystal violet staining in Pdgfra^+/+- and Pdgfra^PI3K/PI3K-derived E13.5 primary MEPM cells grown in medium containing 10% or 0.1% FBS and treated with PDGF-AA for 1 d. Pdgfra^PI3K/PI3K-derived MEPM cells under serum starvation conditions do not proliferate in response to PDGF-AA treatment. Data are presented as mean ± SEM.