Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 May 1;28(9):959–970. doi: 10.1101/gad.236729.113

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Chen and Gartenberg; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 1. — Localization of tDNAs at NPCs. (A) Representative images of cells from asynchronously grown cultures. GFP-lac repressor (green) and Nic96-mRFP (red) are shown in nup133-ΔN and NUP133 cells, outlined in gray. (B) tDNA localization at NPCs. Asynchronously grown cultures of strains MC78 [tT(AGU)C∷256lac^op], MC197 [tS(CGA)C∷256lac^op], MC198 [tT(UGU)G1∷256lac^op], and MC180 (lys2∷256lac^op) were used. N equals the number of cells examined. Localization of each tDNA at NPCs was significantly higher in M phase than in G1 or S. Pairwise χ²-tests, (***) <5 × 10⁻⁴; (**) <5 × 10⁻³. (C) tDNAs colocalized with the nucleolus rarely. Strains MC250 [tT(AGU)C∷256lac^op], MC247 [tS(CGA)C∷256lac^op], MC248 [tT(UGU)G1∷256lac^op], and MC249 (lys2∷256lac^op) bearing a Nop1-CFP expression plasmid (pJW1327) were examined in asynchronous cultures. The specific tDNAs examined here differ from those described in Thompson et al. (2003). (D) NPC-tT(AGU)C colocalization in M phase. Strains MC78 [tT(AGU)C∷256lac^op] and MC180 (lys2∷256lac^op) bearing MET3p-CDC20 were examined before and after depletion of Cdc20. Strains MC64 [tT(AGU)C∷256lac^op] and MC226 (lys2∷256lac^op) were examined before and after addition of nocodazole (NZ). In the absence of either arrest, only M-phase cells were scored. NPC localization was significantly higher for tT(AGU)C than lys2 where noted.