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. 2014 May 1;28(9):959–970. doi: 10.1101/gad.236729.113

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© 2014 Chen and Gartenberg; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 2. — Requirements for NPC–tT(AGU)C contact. (A) Localization of tT(AGU)C in mutants. Strains MC78 (wt), MC210 (Δnup2), MC131 (Δnup60), MC105 [Δtt(agu)c], MC83 (smc3-42), MC82 (mcd1-73), and MC180 (lys2∷256lac^op) were evaluated after Cdc20 depletion. NPC colocalization was significantly lower in mutants. Pairwise χ²-tests, (***) <5 × 10⁻⁴; (**) <5 × 10⁻³; (*) < 5 × 10⁻². (B) Nup60 occupancy at tDNAs. ChIP-qPCR of Nup60-TAP was performed with strains MC177 (wt), MC207 (Δnup2), MC178 (smc3-42), and MC179 (mcd1-73) after arrest in G1 or M phase. (No Ab) No TAP antibody. Nup60 was enriched at tDNAs significantly during M-phase arrest in the wild-type strain but not in mutants (P-values in Supplemental Table S4). (C) Localization of extrachromosomal tT(AGU)C. The half-filled boxes of the diagram represent recombinase target sites that were used to form DNA circles inducibly after arrest in M phase (Dubey and Gartenberg 2007).