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. 2014 May 1;28(9):959–970. doi: 10.1101/gad.236729.113

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© 2014 Chen and Gartenberg; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — tDNA transcription without NPC contact. (A) Flow cytometry of synchronized cell culture. Strain MC230 was released from α-factor arrest at time 0. (B) Unspliced tRNA levels at 15-min intervals after release. Values at 60 min were significantly higher than those at time 0 (P-values are in Supplemental Table S4). (C) Levels of Nup60-TAP binding at representative tDNAs at intervals after release. (D) RNA polymerase III binding in mutants. ChIP-qPCR of Rpc25-TAP at the tDNAs was performed in Cdc20-depleted strains MC195 (wt), MC211 (Δnup2), MC214 (smc3-42), and MC212 (mcd1-73). Rpc25 occupancy was not altered in these mutants.