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. 2014 May 1;28(9):959–970. doi: 10.1101/gad.236729.113

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© 2014 Chen and Gartenberg; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 5. — tDNA transcription and NPC contact in the absence of MAF1. (A) Rpc25 occupancy. ChIP-qPCR of Rpc25-TAP at representative tDNAs was evaluated during M and G1 arrest in strains MC195 (wt) and MC217 (Δmaf1). Rpc25 binding was enhanced significantly in the maf1-null (P-values are in Supplemental Table S4). (B) Localization of tT(AGU)C at NPCs. Asynchronous cultures of MC78 (wt) and MC208 (Δmaf1) were used, as in Figure 1, A and B. Colocalization was significantly higher in the maf1 strain in G1 and S phase. Pairwise χ²-tests, (***) <5 × 10⁻⁴; (**) <1 × 10⁻³. (C) Nup60 binding at tDNAs. ChIP-qPCR of Nup60-TAP was performed in strains MC177 (wt) and MC213 (Δmaf1) during arrest in M or G1 phase. Nup60 binding was enriched significantly in the maf1 mutant in G1.