Skip to main content
. Author manuscript; available in PMC: 2014 May 13.
Published in final edited form as: Nat Immunol. 2012 Sep 30;13(11):1045–1054. doi: 10.1038/ni.2426

Figure 3.

Figure 3

PTEN regulates LPS-induced TIRAP degradation and TLR4 internalization in BMDCs, but SHIP does not. (a) Immunoblot analysis of phosphorylated and total Akt in wild-type, Pten+/− and SHIP-1-deficient (Inpp5d−/−) BMDCs treated for 0–60 min (below lanes) with LPS (100 ng/ml). (b) Immunoblot analysis of TIRAP and α-tubulin (loading control) in lysates of wild-type, Pten+/− and SHIP-1-deficient BMDCs treated for 0–60 min (below lanes) with LPS. (c) Surface expression of TLR4 on wild-type, Pten+/− and SHIP-1-deficient BMDCs stimulated for 0–120 min (horizontal axis) with LPS (100 ng/ml), analyzed by flow cytometry. *P < 0.01 (Student’s t-test). Data are from one experiment representative of three (a), one experiment representative of two with three to four mice per group (b) or three experiments (c; mean ± s.d. of three mice per group).