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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: J Immunol. 2014 Apr 9;192(10):4795–4803. doi: 10.4049/jimmunol.1302764

Figure 2. Human resistin diminished AMPK activity and increased neutrophil sensitivity to LPS challenge.

Figure 2

(A) Levels of TNF-α and MIP-2 were determined in the culture media of hRTN−/−/− and hRTN+/−/− neutrophils. Cells were incubated with LPS (30 ng/ml) for 4 hours and media were subjected to ELISA (Means ± SD, n = 3, *** P < 0.001 compared to untreated or * P < 0.05 compared LPS-treated hRTN−/−/− and hRTN+/−/−). Panel (B) shows amount of TNF-α in culture media obtained from hRTN+/−/− or RTN−/−/− neutrophil that were pretreated dose-dependently with AICAR for 60 minutes followed by exposure to LPS (0 or 30 ng/ml) for additional 4.5 hours. Mean ± SD, n = 4, * P < 0.05. (C and D) Representative Western blots and optical densitometry show phopho-Thr172-AMPK, AMPK, and β-actin obtained from hRTN+/−/− or RTN−/−/− neutrophils treated with AICAR (0, 0, 0.1 or 0.3 mM) for 60 minutes. Mean ± SEM, n = 3 - 4, * P < 0.05.