Table A.3.
Sequences, efficiency and references of primers used for quantitative real-time PCR.
| Target | Primer name | Sequence (5′–3′) | Efficiency (%) | Reference |
|---|---|---|---|---|
| F. graminearum | Fg16NF Fg16NR |
ACAGATGACAAGATTCAGGCACA TTCTTTGACATCTGTTCAACCCA |
97% | Nicholson et al. (1998), Brandfass and Karlovsky (2008) |
| F. culmorum | Fc01F Fc01R |
ATGGTGAACTCGTCGTGGC CCCTTCTTACGCCAATCTCG |
100% | Nicholson et al. (1998) |
| F. langsethiae | FlangF3 LanspoR1 |
CAAAGTTCAGGGCGAAAACT TACAAGAAGACGTGGCGATAT |
97% | Wilson et al. (2004), Edwards et al. (2012) |
| F. avenaceum | Fave574 fwd Fave627 rev |
TATGTTGTCACTGTCTCACACCACC AGAGGGATGTTAGCATGATGAAG |
103% | Nicolaisen et al. (2009) |
| F. tricinctum | Ftri573 fwd Ftri630 rev |
TTGGTATGTTGTCACTGTCTCACACT T TGACAGAGATGTTAGCATGATGCA |
99% | Nicolaisen et al. (2009) |
| F. poae | FpoaeA51 fwd FpoaeA98 rev |
ACCGAATCTCAACTCCGCTTT GTCTGTCAAGCATGTTAGCACAAGT |
99% | Nicolaisen et al. (2009) |
| F. sporotrichioides | Fspor F1 LansporR1 |
CGCACAACGCAAACTCATC TACAAGAAGACGTGGCGATAT |
95% | Wilson et al. (2004), Edwards et al. (2012) |
| M. nivale | Mniv1f Mniv1r |
TTGGCTTGCACAAACAATACTTTTT AGCACAACAGGCGTGGATAAG |
99% | Nielsen et al. (2013) |
| M. majus | Mmajus1f Mmajus1r |
AACCCCTCCCGGGTCAG GGATAAACGACACTTGAAGACAGAAAA |
99% | Nielsen et al. (2013) |