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. Author manuscript; available in PMC: 2015 Mar 31.
Published in final edited form as: Curr Biol. 2014 Mar 13;24(7):760–765. doi: 10.1016/j.cub.2014.02.025

Figure 2. RIC-7 co-localizes with mitochondria and is required for mitochondrial distribution in axons.

Figure 2

(A) Tagged RIC-7 expressed in GABA neurons (Punc-47:RFP::RIC-7, oxEx1598) rescues ric-7 mutants and colocalizes with mitochrondria tagged with Tom20::GFP (‘mito::GFP’). Worms were imaged with the dorsal nerve cord facing the objective. See also Figure S2A,B. (B) Mitochondria tagged with Tom20::GFP are trapped in the cell body and absent from axons in ric-7 and kinesin-1/unc-116 mutants but not kinesin-3/unc-104 mutants. Arrowheads indicate cell bodies, and arrows point to axonal mitochondria. Asterisks indicate gut autofluorescence. See also Figure S2C,D. Worms were imaged with the ventral nerve cord facing the objective. All scale bars are 10 μm. (C) Average number of mitochondria per 50 μm in the ventral nerve cord of GABA motor neuron axons +/− SEM. Mitochondrial distribution is rescued cell autonomously in axons when RFP::RIC-7 is expressed from an array (‘RIC-7(+)’, oxEx1598). p < 0.0001 Kruskal-Wallis test with Dunn’s multiple comparisons to the wild type (black) and to ric-7(n2657) (red) *** p<0.001, * p<0.05. The number of animals is displayed below.