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. Author manuscript; available in PMC: 2014 May 13.
Published in final edited form as: Prostate. 2013 Jun 13;73(14):1507–1517. doi: 10.1002/pros.22698

Figure 4.

Figure 4

Expression of FoxO3a and its binding in TRAMP mice and non-transgenic littermates. (A) Paraffin-embedded (4.0μm) sections from 8, 14, 20 and 28 weeks old mice prostate were used for FoxO3a expression by immunohistochemistry. A strong nuclear and cytoplasmic staining was observed in TRAMP mouse prostate with progressive increase in disease progression where as strong nuclear FoxO3a expression was observed in non-transgenic mice. Magnified at x20 (B) FoxO3a nuclear DNA binding in the dorso-lateral prostates of TRAMP mice and non-transgenic from 8–28 weeks. A progressive decrease in FoxO3a DNA binding was observed in TRAMP mice at 20 and 28 weeks of age. **P<0.001; compared to non-transgenic littermate. Con, Control; Comp, Competitor. Details are described in materials and methods.