Fig. 4.

Overexpression of PRMT5 causes enrichment of EBNA2 occupancy on target promoters. The amount of PRMT5 expressed in each shRNA knockdown cell line was determined by immunoblot analysis. The expression level of PRMT5 in each shRNA expressing cell line is expressed relative to the control group (A). Actin was used as the loading control. Transfection-mediated reporter assays were performed on BJAB cells and the shRNA-expressing cell lines as described in Fig. 3A. The EBNA2-induced Cp-Luc reporter activity in each cell line was expressed relative to the Cp-Luc activity with EBNA2 alone that was observed in BJAB cells (B). ChIP assays were performed on BJAB cells (1 × 10⁷) that were co-transfected with the EBNA2 expression vector, LMP1-Luc reporter plasmid, and either the FPRMT5 or 368R/A expression vector using M2 or EBNA2 antibodies. The abundance of each assayed protein or IgG control on the LMP1 promoter (C upper panel) or GAPDH promoter (C lower panel) is shown. P* value >0.05. P** value<0.05.