PBMCs from young and older adults were stained with antibodies to CD14, CD16, IFN-γR1, R2 or isotype control. The expression of IFN-γR1 and R2 on monocytes (CD14+CD16−) was measured by flow cytometry. (A) Representative histograms showing the expression of IFN-γR1 on monocytes. (B) Mean fluorescent intensity (MFI) of IFN-γR1 expression on monocytes in young and older adults. (C) Representative histograms showing the expression of IFN-γR2 on monocytes. (D) MFI of IFN-γR2 expression on monocytes in young and older adults. Data from 17 young and 17 older adults for B, and 14 young and 9 older adults for D. Bars and error bars indicate mean and standard error of the mean (SEM), respectively. P values were obtained by the Student’s t test.