Skip to main content
. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Clin Immunol. 2014 Mar 19;152(0):101–110. doi: 10.1016/j.clim.2014.03.003

Figure 4. Monocytes of older adults have enhanced expression of interferon regulatory factor 1 with increased IL15 gene expression in response to IFN-γ compared to monocytes of young adults.

Figure 4

Monocytes (CD14+CD16−) were negatively purified from PBMCs of young and older adults using a commercially available kit. Purified cells were stimulated for 4 (A) or 16 (B) hours with IFN-γ (20 ng/ml) or control (PBS). (A) Analysis of IRF1 gene expression by qPCR (young = 6 and older = 10). (B) Analysis of IRF1 protein expression by Western blot. (C) Analysis of IL15 gene expression by qPCR (young = 8 and older = 7). (D) Analysis of IL15RA gene expression (young = 5 and older = 9) by qPCR. Bars and error bars indicate mean and standard error of the mean (SEM), respectively. P value was obtained by the Student’s t test.